Comparison of three different dosages of low-level laser therapy on expression of cell proliferation and inflammatory markers following ovariohysterectomy in rats
Date
Eylül 2023Author
Polat, BülentOkur, Damla Tuğçe
Çolak, Armağan
Okur, Sıtkıcan
Özkaraca, Mustafa
Yılmaz, Kader
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Show full item recordAbstract
The objective of the current study was to evaluate Low-level laser therapy (LLLT) on the healing of
incisional wounds following ovariohysterectomy in rats, by means of subjective histopathological
and immunohistochemical analysis. A total of 72 female Wistar rats were categorised into four
treatment groups (Group I; sacrification 4hours following only one LLLT application, Group II;
sacrification 7days following only one LLLT application, Group III; sacrification 4hours after two LLLT
applications, and Group IV; sacrification 7days after two LLLT applications). Each group was further
divided into four different doses subgroups (Group Control [C, off mode LLLT application], L1 [1 J/
cm2
], L3 [3 J/cm2
], and L6 [6 J/cm2
]), with equal representation in each subgroup. Ovariohysterectomy
was employed using two 2-cm-length midline abdominal incisions in the left and right sides of line
alba. The Group C was assigned to the left side incision to each rat in the study. After irradiation,
the tissue was subjected to histopathological analysis to determine the extent of mononuclear cell
infiltration, edoema, and epithelialization. Additionally, immunohistochemical analysis was performed
to evaluate the expression of proliferating cell nuclear antigen (pCNA) and inducible nitric oxide
synthase (iNOS). Group L1 and L3 significantly decreased mononuclear cell infiltration compared with
Group C in all treatment groups (p<0.05). Group L3 significantly decreased edoema compared with
Group C in all groups except for treatment Group I (p<0.05). Group L2 and L3 significantly increased
epithelization in treatment Group IV (p<0.05). Moreover, Group L2 and L3 significantly increased
pCNA in all groups, while L2 and L3 significantly decreased iNOS expression in treatment Group II,
III, and IV (p<0.05). However, no statistical difference was found between subgroups of treatment
Group I in iNOS expiration (p>0.05). The results of the current examination demonstrated that LLLT
can modulate mononuclear cell infiltration and edoema, and improve epithelization, as well as
increase pCNA expression, whereas decrease iNOS expression during the wound healing process,
therefore enhancing wound healing following ovariohysterectomy in rats