The investigation of host genetic variants of toll-like receptor 7 and 8 in COVID-19
Abstract
Toll-like receptors (TLRs) recognize infectious agents and play an important role in the innate immune system. Studies have suggested that TLR single nucleotide polymorphisms (SNPs) are associated with poor antiviral responses against SARS-CoV-2. Therefore, we aimed to investigate the relationship of TLR7 and TLR8 (SNPs) with COVID-19 disease prognosis. A total of 120 COVID-19 patients, 40 outpatients, 40 clinical ward patients and 40 intensive care unit (ICU) patients were included in the study. TLR7 (rs179009), TLR8-129 C/G (rs3764879) and TLR8 Met1Val (rs3764880) SNPs were genotyped using the PCR-RFLP method. In female patients, individuals carrying AG genotype and G allele for TLR8 Met1Val SNP were found at a higher frequency in patients hospitalized in the ICU than in patients followed in the clinical ward (p < 0.05). In terms of the other two SNPs, no significant difference was found between the groups in females. Furthermore, in male patients, A allele of TLR7 rs179009 SNP was at a higher frequency in patients who have at least one comorbidity than in patients who have no comorbidity (p < 0.05). Our results suggest that TLR8 Met1Val SNP is important in the COVID-19 disease severity in females. Furthermore, TLR7 rs179009 SNP is important in male patients in the presence of comorbid diseases. Toll-like receptors (TLRs) recognize infectious agents and play
an important role in the innate immune system. Studies have
suggested that TLR single nucleotide polymorphisms (SNPs)
are associated with poor antiviral responses against SARSCoV-
2. Therefore, we aimed to investigate the relationship of
TLR7 and TLR8 (SNPs) with COVID-19 disease prognosis. A total
of 120 COVID-19 patients, 40 outpatients, 40 clinical ward
patients and 40 intensive care unit (ICU) patients were included
in the study. TLR7 (rs179009), TLR8-129 C/G (rs3764879) and
TLR8 Met1Val (rs3764880) SNPs were genotyped using the
PCR-RFLP method. In female patients, individuals carrying AG
genotype and G allele for TLR8 Met1Val SNP were found at a
higher frequency in patients hospitalized in the ICU than in
patients followed in the clinical ward (p < 0.05). In terms of the
other two SNPs, no significant difference was found between
the groups in females. Furthermore, in male patients, A allele
of TLR7 rs179009 SNP was at a higher frequency in patients
who have at least one comorbidity than in patients who have
no comorbidity (p < 0.05). Our results suggest that TLR8 Met1Val
SNP is important in the COVID-19 disease severity in females.
Furthermore, TLR7 rs179009 SNP is important in male patients
in the presence of comorbid diseases.