Investigation of the effect of sugammadex on glutamate-induced neurotoxicity in C6 cell line and the roles played by nitric oxide and oxidative stress pathways

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dc.authoridJoha, Ziad/0000-0001-8520-3760
dc.contributor.authorDirik, Hasan
dc.contributor.authorJoha, Ziad
dc.date.accessioned2024-10-26T18:04:13Z
dc.date.available2024-10-26T18:04:13Z
dc.date.issued2023
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThis experiment was intended to evaluate the effect of sugammadex on the cytotoxicity induced by glutamate, involving the nitric oxide and oxidative stress pathways. C6 glioma cells were used in the study. Glutamate was given to cells in the glutamate group for 24 h. Sugammadex at different concentrations was given to cells in the sugammadex group for 24 h. Cells in the sugammadex + glutamate group were pre-treated with sugammadex at various concentrations for 1 h and then exposed to glutamate for 24 h. XTT assay was used to assess cell viability. Levels of nitric oxide (NO), neuronal nitric oxide synthase (nNOS), total antioxidant (TAS), and total oxidant (TOS) in the cells were calculated using commercial kits. Apoptosis was detected by TUNEL assay. Sugammadex at concentrations of 50 and 100 mu g/mL significantly enhanced the cell viability in C6 cells after the cytotoxicity induced by glutamate (p < 0.001). Moreover, sugammadex considerably decreased the levels of nNOS NO and TOS and the number of apoptotic cells and increased the level of TAS (p < 0.001). Sugammadex has protective and antioxidant properties on cytotoxicity and could be an effective supplement for neurodegenerative diseases such as Alzheimer and Parkinson if further research in vivo supports this claim.
dc.identifier.doi10.1111/fcp.12890
dc.identifier.endpage793
dc.identifier.issn0767-3981
dc.identifier.issn1472-8206
dc.identifier.issue4
dc.identifier.pmid36880372
dc.identifier.scopus2-s2.0-85150733957
dc.identifier.scopusqualityQ2
dc.identifier.startpage786
dc.identifier.urihttps://doi.org/10.1111/fcp.12890
dc.identifier.urihttps://hdl.handle.net/20.500.12418/28810
dc.identifier.volume37
dc.identifier.wosWOS:000950356000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofFundamental & Clinical Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectC6 rat glioma
dc.subjectglutamate
dc.subjectnitric oxide
dc.subjectoxidative stress
dc.subjectsugammadex
dc.titleInvestigation of the effect of sugammadex on glutamate-induced neurotoxicity in C6 cell line and the roles played by nitric oxide and oxidative stress pathways
dc.typeArticle

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