Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines

Durna Daştan, Sevgi

Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines

dc.authorid	0000-0003-4946-5602	tr
dc.contributor.author	Durna Daştan, Sevgi
dc.date.accessioned	2023-04-13T13:39:06Z
dc.date.available	2023-04-13T13:39:06Z
dc.date.issued	2022	tr
dc.department	Fen Fakültesi	tr
dc.description.abstract	AbstractNano-biotechnology-based clinical applications to cure health-related issues have gained huge attention among the scientific community and hold great promise to limit cancer metastasis. In this study, green-derived silver nanoparticles were synthesized by using leaf extract of Litchi chinensis. Characterization of biosynthesized silver nanoparticles was performed by using UV–Vis spectroscopy, FTIR, XRD, EDS, and SEM analysis. The clinical application of green-drive nanoparticles was investigated by using MCF-7 cancer cell lines. MCF-7 breast cancer cell lines were analyzed against three different treatments. (i) Silver nanoparticles (AgNPs), (ii) miR34a mimics and (iii) Co-delivery of AgNPs and miR34a mimics. Cell viability was determined by MTT assay and, extraction of mRNA and cDNA synthesis were performed after successful cellular transfection. qRT-PCR was done for expression analysis of DR4 and DR5 upon exogenous delivery of all 3 treatments. Results indicate that L. chinensis leaves have a significant amount of phenolic and flavonoid contents and also possess massive antioxidant activity. The diameter of nanoparticles was observed in the range of 41–55 nm. It was concluded that green-derived silver nanoparticles can be a potential contributing agent for cancer prevention and are reported to upregulate the expression of DR4 and DR5 by 0.8-folds and 3.7-folds, respectively.	tr
dc.identifier.citation	Muhammad Javed Iqbal1,2, Umer Rashid1, Zeeshan Javed3, Zara Hamid1 , Komal Imran2 , Ayesha Kabeer2 Shahid Raza3 , Zainab M. Almarhoon4 , Željko Reiner5 , Iulia‑Cristina Bagiu6,7, Radu Vasile Bagiu8 , Ioan Sarac9 Javad Sharif‑Rad10, Alibek Ydyrys11, Sevgi Durna Daştan12,13, Monica Butnariu9 and William C. Cho14*	tr
dc.identifier.doi	10.1186/s12645-022-00137-8	en_US
dc.identifier.endpage	20	tr
dc.identifier.issue	13	tr
dc.identifier.scopus	2-s2.0-85139670003	en_US
dc.identifier.scopusquality	N/A
dc.identifier.startpage	1	tr
dc.identifier.uri	http://dx.doi.org/10.1186/s12645-022-00137-8
dc.identifier.uri	https://hdl.handle.net/20.500.12418/13686
dc.identifier.volume	ekim 2022	tr
dc.identifier.wos	WOS:000866119200001	en_US
dc.identifier.wosquality	Q2
dc.indekslendigikaynak	Web of Science	en_US
dc.indekslendigikaynak	Scopus	en_US
dc.language.iso	en	en_US
dc.publisher	cancer nanotechnology	tr
dc.relation.ispartof	cancer nanotechnology	en_US
dc.relation.publicationcategory	Uluslararası Editör Denetimli Dergide Makale	tr
dc.rights	info:eu-repo/semantics/openAccess	tr
dc.subject	MCF-7 cell lines, Silver nanoparticles synthesis, DR4, DR5, Breast cancer	tr
dc.title	Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines	en_US
dc.type	Article	en_US