Investigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method

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dc.authoridOZSENSOY, Yusuf/0000-0002-2605-2410
dc.authoridKARA, Haki/0000-0002-0295-0582
dc.contributor.authorOzsensoy, Yusuf
dc.contributor.authorKara, Haki
dc.date.accessioned2024-10-26T18:00:04Z
dc.date.available2024-10-26T18:00:04Z
dc.date.issued2019
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractThe Anatolian water buffalo is a native water buffalo breed only being reared in Turkey. The objective of this study was to investigate gene polymorphisms on exons 4 and 5 of the growth hormone (GH) and on exon 10 of the growth hormone receptor (GHR), which are thought to be related to meat yields of Anatolian water buffalo. Blood samples of 192 water buffaloes from Sivas Province were used in DNA extractions by phenol-chloroform method. DNA samples were amplified by using specific pruners in PCR. PCR products were digested by Alu I (GH, n: 167) and Alu I (GHR, n: 192) restriction enzymes in order to determine polymorphisms. Digested PCR products were separated in 2.5%-3% agarose gel electrophoresis to determine allelic polymorphisms. As a result, the LL (78.44%) and LV (21.56%) genotypes and L (0.89) and V (0.11) alleles for the GH gene and the AA (7.81%) and AG (92.19%) genotypes and A (0.54) and G (0.46) alleles for the GHR gene were obtained. Gene polymorphisms were not detected (P > 0.05) on the Gil gene, but a significant difference was found for the GHR gene (P < 0.001). Therefore, it can be said that Anatolian water buffaloes have increased meat yield due to the presence of genotypes in gene regions. This was the first investigation of enzyme polymorphisms of the GHR gene in Anatolian water buffaloes.
dc.description.sponsorshipScientific Research Projects Fund of Sivas Cumhuriyet University [V-051]
dc.description.sponsorshipThis research was supported by the Scientific Research Projects Fund of Sivas Cumhuriyet University under Project Number V-051.
dc.identifier.doi10.3906/zoo-1907-44
dc.identifier.endpage565
dc.identifier.issn1300-0179
dc.identifier.issn1303-6114
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85074669200
dc.identifier.scopusqualityQ3
dc.identifier.startpage560
dc.identifier.trdizinid335706
dc.identifier.urihttps://doi.org/10.3906/zoo-1907-44
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/335706
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27501
dc.identifier.volume43
dc.identifier.wosWOS:000495863700003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Zoology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGrowth hormone
dc.subjectgrowth hormone receptor
dc.subjectPCR-RFLP
dc.subjectSivas
dc.subjectwater buffalo
dc.titleInvestigation of GH and GHR Alu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method
dc.typeArticle

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