Evaluation of the Neurotoxicity of Strontium and Glycyrrhiza Glabra: First Report

AIM: To investigate the neurotoxic effects of strontium (Sr) compounds and Glycyrrhiza glabra (licorice, G. glabra). MATERIAL and METHODS: In this study, we conducted neurotoxicity assays on the human cortical neuronal cell line HCN-2 (CRL-10742) to determine the potential neurotoxic effects of Sr and G. glabra. RESULTS: No significant decrease in HCN-2 cell viability was observed with longer Sr exposure or Sr concentrations up to 2000 mu g/mL. The IC50 values of Sr for 24 and 48 hours of exposure were >2000 mu g/mL, and 936.9 +/- 0.09 mu g/mL for 72 hours. However, we observed a significant reduction in HCN-2 cell viability with longer exposure and higher concentrations of G. glabra. The IC50 values of G. glabra for 24, 48, and 72 hours were 545.1 +/- 0.03 mu g/mL, 398.1 +/- 0.03 mu g/mL, and 393.3 +/- 0.02 mu g/mL, respectively. CONCLUSION: Additional studies are needed to further investigate the neurotoxicity of Sr and G. glabra, and elucidate the pathway by which these compounds exert their therapeutic effects in pathological conditions.