The Effects of Tofacitinib-Mediated Janus Kinase/Signal Transducers and Activators of the Transcription Signal Pathway Inhibition on Collagen Biosynthesis in Hepatic and Skin Fibroblast Celt Culture

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dc.authoridAltun, Ahmet/0000-0003-2056-8683
dc.authoridSAHIN, ALI/0000-0002-6953-4276
dc.authoridAydin, Huseyin/0000-0002-3194-830X
dc.authoridsahin, ali/0000-0003-0743-5288
dc.contributor.authorSahin, Mehtap
dc.contributor.authorAydin, Huseyin
dc.contributor.authorAltun, Ahmet
dc.contributor.authorDerin, Mehmet Emin
dc.contributor.authorSahin, Ali
dc.date.accessioned2024-10-26T18:00:07Z
dc.date.available2024-10-26T18:00:07Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractObjectives: This study aims to investigate the effects of Janus kinase/signal transducers and activators of the transcription (JAK/STAT) pathway inhibition on collagen biosynthesis in fibroblast cell culture by tofacitinib. Materials and methods: BJ-CRL-1474 (R) (skin) and BRL3A (R) (hepatic) fibroblast cell cultures were proliferated in a suitable medium. Tofacitinib was administered to fibroblast cells proliferating in 96-well flasks at concentrations of 25, 50, 100, 200, 400, and 800 nM. Tissue inhibitor of metalloproteinase-1 (TIMP-1), matrix metalloproteinase-3 (MMP-3), transforming growth factor beta 1 (TGF-beta 1), and hydroxyproline levels were measured using the enzyme-linked immunosorbent assay method. Results: Tofacitinib showed cytotoxic effect on skin and liver cell culture. The cytotoxic effect of tofacitinib started at 100 nM (p<0.05). The highest effect was obtained at 800 nM. The time-dependent cytotoxic effect of tofacitinib was significantly higher at all concentrations after 72 hours than at 24 and 48 hours (p<0.05). The level of TGF-beta 1 was significantly lower even at a tofacitinib concentration of 25 nM (p<0.05). There were significant decreases in MMP-3, TIMP-1, and hydroxyproline levels after tofacitinib administration (p<0.05). Conclusion: Tofacitinib inhibited fibroblast cell proliferation in a concentration-dependent manner in a fibroblast cell culture. However, further extensive animal and human studies are necessary to determine the clinical significance of this effect.
dc.description.sponsorshipCumhuriyet University Scientific Research Projects (CUBAP) Commission [T-714]
dc.description.sponsorshipThis study was supported by Cumhuriyet University Scientific Research Projects (CUBAP) Commission (Project Number: T-714).
dc.identifier.doi10.46497/ArchRheumatol.2020.7568
dc.identifier.endpage350
dc.identifier.issn2148-5046
dc.identifier.issn1309-0283
dc.identifier.issue3
dc.identifier.pmid33458657
dc.identifier.scopus2-s2.0-85091723411
dc.identifier.scopusqualityQ4
dc.identifier.startpage343
dc.identifier.trdizinid364199
dc.identifier.urihttps://doi.org/10.46497/ArchRheumatol.2020.7568
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/364199
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27538
dc.identifier.volume35
dc.identifier.wosWOS:000574427300004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish League Against Rheumatism
dc.relation.ispartofArchives of Rheumatology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCollagen
dc.subjectfibroblast cell culture
dc.subjectJanus kinase/signal transducers and activators of the transcription
dc.subjecttofacitinib
dc.titleThe Effects of Tofacitinib-Mediated Janus Kinase/Signal Transducers and Activators of the Transcription Signal Pathway Inhibition on Collagen Biosynthesis in Hepatic and Skin Fibroblast Celt Culture
dc.typeArticle

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