Vortex-assisted dispersive solid-phase microextraction of manganese in food samples by using newly synthesized biodegradable poly (3-hydroxy butyrate) diethanol amine

A newly synthesized biodegradable polyhydroxy butyrate diethanol amin (PHB-DEA) was used as adsorbent for vortex assisted dispersive solid phase microextraction (VA-dSP mu E) of manganese in food samples. The different parameters were optimized such as pH, amount of adsorbent, time, desorption solvents and also number of reuses. After the sample preparation step, the determination of manganese from food samples was carried out using flame atomic absorption spectrometry. The present developed method showed good LOD value 0.15 ng mL(-1) and LOQ 0.5 ng mL(-1). The 0.5-300 ng mL(-1) linearity range was found and enhancement factor was 182. The inter-day RSD% was 2.2-4.1 and intra-day RSD% was 2.8-5.2. The recovery of manganese was found in 95-98 % . The factorial design was employed such as response surface plots, pareto chart, residual plots and ANOVA to estimate the significant level of the model using central composite design (CCD). The developed VA-dSP mu E procedure was successfully implemented to determine manganese in food samples.