In vitro antimicrobial, antioxidant, cytotoxic activities, and wound healing potential of Thymbra capitata ethanolic extract

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dc.authoridTUNC, Tutku/0000-0002-8274-9386
dc.authoridhepokur, ceylan/0000-0001-6397-1291
dc.contributor.authorHepokur, Ceylan
dc.contributor.authorMisir, Sema
dc.contributor.authorTunc, Tutku
dc.contributor.authorTutar, Ugur
dc.contributor.authorHepokur, Ali Ihsan
dc.contributor.authorCicek, Mehmet
dc.date.accessioned2024-10-26T18:00:19Z
dc.date.available2024-10-26T18:00:19Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractObjectives: In this study, we aimed to detect the chemical compounds of Thymbra capitata ethanolic extract (TC-EtOH) as well as to evaluated its antimicrobial, antioxidant, cytotoxic activities, and in vivo wound healing effects. Methods: The chemical composition of TC-EtOH was analyzed by Gas chromatography-mass spectrometry (GC-MS). Antioxidant and antimicrobial properties were determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH), disc diffusion test and broth micro-dilution (minimal inhibitory concentration [MIC]) methods, respectively. Cytotoxic activity was tested on MG63 (human osteosarcoma) and MCF-7 (human breast carcinoma) cells by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. Tumor necrosis factor alpha (TNF-alpha) protein levels were determined by ELISA. Results: The major components of TC-EtOH were tetratria contane (14.92%), camphor (12.50%), and terpineol (10.77%). TC-EtOH showed powerful antimicrobial activity in C. Tropicalis (0.03 mg/mL). The IC50 values of the TC-EtOH of the DPPH were determined 21.5 mu g/mL. The IC50 values were calculated 37.28 and 44.40 mu g/mL on the MG63 and MCF-7 cell lines, respectively. It was observed that the wounds treated with TC-EtOH showed a faster healing. Conclusions: According to results, T. capitata species are thought to be natural antioxidants and a novel pharmaceutical compound for the pharmaceutical industry.
dc.identifier.doi10.1515/tjb-2019-0470
dc.identifier.endpage849
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85098958602
dc.identifier.scopusqualityQ4
dc.identifier.startpage843
dc.identifier.trdizinid455043
dc.identifier.urihttps://doi.org/10.1515/tjb-2019-0470
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/455043
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27635
dc.identifier.volume45
dc.identifier.wosWOS:000603517600025
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectantimicrobial
dc.subjectantioxidant
dc.subjectcytotoxic activity
dc.subjectThymbra capitata
dc.subjectwound healing
dc.titleIn vitro antimicrobial, antioxidant, cytotoxic activities, and wound healing potential of Thymbra capitata ethanolic extract
dc.typeArticle

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