The effects of specific and non-specific phosphodiesterase inhibitors and N-acetylcysteine on oxidative stress and remote organ injury in two-hit trauma model

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dc.authoridtopal, ugur/0000-0003-1305-2056
dc.contributor.authorOzer, Ozdemir
dc.contributor.authorTopal, Ugur
dc.contributor.authorSen, Metin
dc.date.accessioned2024-10-26T18:00:08Z
dc.date.available2024-10-26T18:00:08Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractBACKGROUND: Sepsis is a systemic inflammatory response to infection and is one of the leading causes of morbidity and mortality. The second hit after trauma causes increased inflammatory response and multiple organ failure (MOF). The infection which develops after burn injury is a suitable model for a two-hit trauma study. Sepsis causes the release of biochemical mediators, such as Free Oxygen Radicals (FORs), which may lead to lipid peroxidation, which may play a key role in multiple organ failure. In this study, we aimed to investigate the effects of phosphodiesterase (PDE) inhibitors (sildenafil, milrinone, pentoxifylline) and N-acetylcysteine (NAS) on oxidative stress and organ damage in two-hit models. METHODS: In this experimental study, peritonitis was created by cecal ligation and puncture (CLP) method in 40 rats, 72 hours after creating a 30% scalding injury. Rats were divided into five groups of eight rats each as follows: Group I: No treatment; Group II: 10/mg/kg/day dosage of intraperitoneal (i.p) sildenafil treatment was applied for 72 hours after CLP; Group III: 1/mg/kg/day dosage of i.p milrinone treatment was applied for 72 hours after CLP; Group IV: 150/mg/kg/day dosage of i.p NAS treatment was applied for 72 hours after CLP; Group V: 50/mg/kg/day dosage of i.p pentoxifylline treatment was applied for 72 hours after CLP. All rats were sacrificed on the seventh day of this study. Malondialdehyde (MDA), Glutathione Peroxidase (GPx), Superoxide Dismutase (SOD), catalase, Tumor Necrotic Factor-alpha (TNF-alpha) levels, and tissue (lung, kidney) and serum samples were taken for histopathological study. RESULTS: When compared to the control group, the tissue damage score was found to be lower in all treatment groups. Sildenafil, milrinone and NAS groups had higher kidney GPx levels compared to the control group. Milrinone and pentoxifylline were higher in the lung tissue compared to the SOD control group. TNF alpha levels were lower in pentoxifylline and milrinone groups compared to the control group. CONCLUSION: This experimental study has shown that PDE inhibitors and NAS have a decreasing effect on oxidative stress and distant organ damage in the two-hit model. Further clinical and experimental studies are needed on this subject.
dc.description.sponsorshipCumhuriyet University scientific research projects commission [T544]
dc.description.sponsorshipThis project was supported by Cumhuriyet University scientific research projects commission as T544.
dc.identifier.doi10.14744/tjtes.2019.00570
dc.identifier.endpage525
dc.identifier.issn1306-696X
dc.identifier.issn1307-7945
dc.identifier.issue4
dc.identifier.pmid32589233
dc.identifier.scopus2-s2.0-85087127470
dc.identifier.scopusqualityQ2
dc.identifier.startpage517
dc.identifier.trdizinid367016
dc.identifier.urihttps://doi.org/10.14744/tjtes.2019.00570
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/367016
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27543
dc.identifier.volume26
dc.identifier.wosWOS:000580593100004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Assoc Trauma Emergency Surgery
dc.relation.ispartofUlusal Travma ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntioxidant
dc.subjectfree oxygen radicals
dc.subjecttissue damage
dc.subjectphosphodiesterase inhibitor
dc.subjectsepsis
dc.titleThe effects of specific and non-specific phosphodiesterase inhibitors and N-acetylcysteine on oxidative stress and remote organ injury in two-hit trauma model
dc.typeArticle

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