Research into biocompatibility and cytotoxicity of daptomycin, gentamicin, vancomycin and teicoplanin antibiotics at common doses added to bone cement

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dc.authoridTUNC, Tutku/0000-0002-8274-9386
dc.contributor.authorKilinc, Seyran
dc.contributor.authorTunc, Tutku
dc.contributor.authorPazarci, Ozhan
dc.contributor.authorSumer, Zeynep
dc.date.accessioned2024-10-26T18:00:15Z
dc.date.available2024-10-26T18:00:15Z
dc.date.issued2020
dc.departmentSivas Cumhuriyet Üniversitesi
dc.description.abstractObjectives: This study aims to investigate the biocompatibility and cytotoxicity of daptomycin, gentamicin, vancomycin and teicoplanin at commonly-used dose intervals added to polymethylmethacrylate (PMMA) in vitro . Materials and methods: This prospective study was conducted between February 2016 and June 2016. Antibiotics were added to PMMA at doses frequently used in clinical practice. The antibiotic doses added were teicoplanin (2 g, 3 g, 4 g), gentamicin (0.5 g, 0.75 g, 1 g), daptomycin (0.5 g.) and vancomycin (2 g, 3 g, 4 g). Standard cement balls (10 mm) were created. Activated L929 mouse fibroblast cell culture was used for incubation. Agar diffusion, Cell Proliferation Kit II (XTT) test and electron microscope investigations were performed to examine biocompatibility and cytotoxicity. Results: In the cytotoxicity test, teicoplanin at 4 g and daptomycin at 0.5 g doses were observed to cause reductions in viability percentages. The same doses caused 20% and 20-40% cell lysis indices during the agar diffusion test. On electron microscope images, cytotoxic effects in fibroblast cells and involvement with the surface of cement balls were observed. Conclusion: Gentamicin, vancomycin and teicoplanin were observed to be non-toxic and biocompatible at commonly-used dose intervals. Teicoplanin at 4 g and daptomycin at 0.5 g doses were identified to be cytotoxic and not biocompatible. When selecting antibiotics to be added to bone cement, care should be taken that the antibiotic is non-toxic and biocompatible.
dc.identifier.doi10.5606/ehc.2020.74943
dc.identifier.endpage334
dc.identifier.issn2687-4784
dc.identifier.issn2687-4792
dc.identifier.issue2
dc.identifier.pmid32584733
dc.identifier.scopus2-s2.0-85089543808
dc.identifier.scopusqualityQ2
dc.identifier.startpage328
dc.identifier.trdizinid407698
dc.identifier.urihttps://doi.org/10.5606/ehc.2020.74943
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/407698
dc.identifier.urihttps://hdl.handle.net/20.500.12418/27595
dc.identifier.volume31
dc.identifier.wosWOS:000545984600024
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Joint Diseases Foundation
dc.relation.ispartofJoint Diseases and Related Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbiocompatible
dc.subjectKeywords
dc.subjectAntibiotics
dc.subjectbiocompatibility
dc.subjectbone cement
dc.subjectcytotoxicity
dc.subjectpolymethylmethacrylate
dc.titleResearch into biocompatibility and cytotoxicity of daptomycin, gentamicin, vancomycin and teicoplanin antibiotics at common doses added to bone cement
dc.typeArticle

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