Whole-genome sequence analysis of Salmonella Infantis isolated from raw chicken meat samples and insights into pESI-like megaplasmid

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dc.authorid0000-0002-8173-7818tr
dc.contributor.authorKürekci, Cemil; Sahin, Seyda; Iwan, Ewelina; Kwit, Reneta; Bomba, Arkadiusz; Wasyl, Dariusz
dc.date.accessioned2022-05-10T10:20:20Z
dc.date.available2022-05-10T10:20:20Z
dc.date.issued16.01.2021tr
dc.departmentVeteriner Fakültesitr
dc.description.abstractThere has been an increase in the number of reports on Salmonella enterica subsp. enterica serovar Infantis (S. Infantis) isolated from animals and humans. Recent studies using whole genome sequencing (WGS) have provided evidence on the likely contribution of a unique conjugative megaplasmid (pESI; ~280 kb) to the dissemination of this serovar worldwide. In the present study, twenty-two unrelated Salmonella strains [S. Infantis (n = 20) and Salmonella 6,7:r:- (n = 2)] and their plasmids were investigated using next generation sequencing technologies (MiSeq and MinION) to unravel the significant expansion of this bacteria in Turkey. Multi-locus sequence typing, plasmid replicons, resistance gene contents as well as phylogenetic relations between strains were determined. According to the WGS data, all S. Infantis possessed the relevant megaplasmid backbone genes and belonged to sequence type 32 (ST32) with the exception of a single novel ST7091. Tetracycline and trimethoprim/sulfamethoxazole resistance were found to be widespread in S. Infantis strains and the resistant strains exclusively carried the tetA, sul1, sul2 and dfrA14 genes. One S. Infantis isolate was also a carrier of the plasmid-mediated ampC via blaCMY-2, gene. Moreover, full genomes of four S. Infantis isolates were reconstructed based on hybrid assembly. All four strains contained large plasmids (240–290 kb) similar to previously published megaplasmid (pESI) and accompanied by several small plasmids. The megaplasmid backbone contained a toxin-antitoxin system, two virulence cassettes and segments associated with heavy metals resistance, while variable regions possessed several antibiotic resistance genes flanked by mobile elements. This study indicated that pESI-like megaplasmid is widely disseminated within the tested S. Infantis strains of chicken meat, warranting further genomic studies on clinical strains from humans and animals to uncover the overall emergence and spread of this serovar.tr
dc.identifier.doi10.1016/j.ijfoodmicro.2020.108956en_US
dc.identifier.endpage8tr
dc.identifier.pmid33189985en_US
dc.identifier.scopus2-s2.0-85096017631en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage1tr
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0168160520304505?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.12418/12729
dc.identifier.volume337tr
dc.identifier.wosWOS:000600914600010en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElseviertr
dc.relation.ispartofInternational Journal of Food Microbiolgyen_US
dc.relation.publicationcategoryUluslararası Hakemli Dergide Makale - Kurum Öğretim Elemanıtr
dc.rightsinfo:eu-repo/semantics/openAccesstr
dc.subjectS. Infantis, Chicken meat, Whole genome sequencing, Clonal spread, Megaplasmidtr
dc.titleWhole-genome sequence analysis of Salmonella Infantis isolated from raw chicken meat samples and insights into pESI-like megaplasmiden_US
dc.typeArticleen_US

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